Moreover, the cellular assay determined that propofol led to an increase in the cell mortality, membrane leakage, upregulation of intracellular ROS, elevation of Caspase-3 and -9 protein levels, and overexpression of Bax/Bcl-2 mRNA ratio, in PBMCs. Molecular docking study indicated that formation of IgG-propofol complex occurs through involvement of hydrogen bonding and van der Waals forces. Far- and near-circular dichroism (CD) study further demonstrated that the structure of IgG was unfolded after interaction with propofol. The UV–Vis, intrinsic/ANS/synchronous fluorescence spectroscopic studies indicated the static quenching of IgG and corresponding protein unfolding in the presence of propofol. Therefore, in this study the interaction of propofol with immunoglobulin G (IgG) and peripheral blood mononuclear cells (PBMCs) as a model of blood system was explored by different spectroscopic, theoretical, cellular and molecular assays. However, the side effects of propofol are still elusive in the blood system. Propofol is known as an intravenous anesthetic drug utilized for stimulation and prolongation of general anesthesia.
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